Elements of photochemistry in medicine

Basic knowledge of general and organic chemistry.

(in Polish) przedmiot obowiązkowy

1. 10h lecture, 30h laboratory, 25h consultation, i.e. 65h contact hours

2. 20h individual student work,

3. 15h time required to prepare for participation in the assessment process,

4. total student workload - 100h.

Student:

W1: knows the influence of electromagnetic radiation on matter - K_W01

W2: distinguishes between photophysical and photochemical processes - K_W01

W2: knows the basic photochemical laws - K_W01

W3: defines the quantum yield of energy and electron transfer and quenching processes - K_W01

W4: knows the sources of UV radiation - K_W01, K_W03

Student:

U1: performs simple photochemical experiments - K_U01

U2: correctly interprets the effects of UV radiation on matter - K_U01, K_U03

U3: safely uses UV radiation sources - K_U03

U4: can measure the intensity of UV radiation using chemical actinometers - K_U01

Student:

K1: notices dependencies independently and draws conclusions correctly - K_K01,

K2: works systematically and consistently - K_K01.

K3: knows and complies with the rules and standards applicable in the photochemical laboratory - K_K01,

K4: works in a group - K_03.:

Student:

K1: notices dependencies independently and draws conclusions correctly - K_K01,

K2: works systematically and consistently - K_K01.

K3: knows and complies with the rules and standards applicable in the photochemical laboratory - K_K01,

K4: works in a group - K_03.:

- informative (conventional) lecture

The aim of the course is to familiarize students with the nature and influence of electromagnetic radiation on matter and living organisms, basic photophysical and photochemical processes, and the use of light in science, medicine and pharmacy; photodegradation and photoaging processes of chemical and natural substances (including model compounds of living organisms).

Lecture:

The lecture contains information on the nature of electromagnetic radiation, the interaction of light with matter and its impact on living organisms. Basic concepts and laws of photochemistry, issues related to the absorption of radiation and excited states of molecules and the processes of quenching of excited states, fluorescence and phosphorescence, the Jabłoński diagram, quantum efficiencies, energy and electron transfer processes will be discussed. Examples of photochemical reactions and materials used in medicine (photopolymer implants, prostheses, eye lenses, composite dental fillings, photosensitive compounds in medical diagnostics) and pharmacy (photopolymer drug carriers with controlled release), as well as the use of UV radiation as a factor in photodynamic therapy will be presented. anti-cancer and as a sterilizing agent

Artificial sources of UV radiation, methods of testing photochemical reactions and actinometric methods will be discussed.

Lab:

Laboratory exercises are devoted to the detection of singlet oxygen produced in photochemical reactions, examining the influence of UV radiation on the decomposition of a selected dye in the presence and absence of catalysts, examining photochromic processes in organic compounds, examining photochemical transformations of pyrimidine and purine bases (as model compounds of proteins), examining photosensitization processes and photostabilization.

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1. S. Paszyc, Fundamentals of photochemistry, PWN, Warsaw 1992.

2. J.A. Baltrop, J.D. Coyle, Photochemistry - basics, PWN, Warsaw 1987.

3. P. Suppan, Chemistry and light, PWN, Warsaw 1997.

4. Collective work, J. Pączkowski (ed.), Photochemistry of polymers, Nicolaus Copernicus University Publishing House, Toruń 2003.

5. Collective work, J. Najbar, A. Turek (eds.), Photochemistry and optical spectroscopy. Laboratory exercises, PWN, Warsaw 2009.

6. W. Schnabel, Polymers and Light. Fundamentals and Technical Applications, Wiley-VCH, Weinheim 2007.

List of additional literature:

1. Collective work, B. Marciniak (ed.), Methods of investigating the mechanisms of photochemical reactions, Wydawnictwo UAM, Poznań 1999.

2. G. Wenska, Environmental photochemistry, Wydawnictwo UAM, Poznań 1997.

3. Z. Stasicka, Photochemical processes in the environment, Wydawnictwo UJ, Kraków 2001.

4. J. Guillet, Polymer Photophysics and Photochemistry, Cambridge University Press, Cambridge 1987.

5. N.J.Turro, V. Ramamurthy, J.C. Scaiano, Modern molecular photochemistry of organic molecules, University Science Books, Sausalito 2010.

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Lecture: written exam – W1-W4.

Laboratory: credit based on completed laboratory exercises and studies - W1, W2, W4, U1, U3, U4, U5, K1-K3, continuous assessment of the student's work during classes - U3, U5, K1-K3.

Exam requirements:

1. Electromagnetic radiation, photochemical laws.

2. Processes of absorption, excitation and photosensitization of molecules.

3. Jabłoński's diagram.

4. Excimers and exciplexes.

5. Quantum efficiency.

6. Energy and electron transfer processes.

7. Photochemical reactions, photosensitive compounds.

8. Actinometry.

9. The influence of light on living organisms.

10. The use of UV radiation in medicine and pharmacy.