Gas chromatography and hyphenated techniques

The graduates of natural sciences and life sciences area

compulsory course

Number of lecture hours- 18 h

Number of laboratory classes hours- 20 h

knows the theoretical and practical aspects of the gas chromatography and hyphenated techniques - K_W12.

make GC and GC/MS analyses, can interpret obtained data/results - K_U12.

skills give the possibility to develop of analytical methods and procedures - K_K01.

- lectures as a multimedia presentations;

- one by one and on-line consultations (distance learning);

- laboratory exercises instructions prepared based on the information giving during the lectures are useful and required at the laboratory;

- individual work with the apparatus and advanced analytical equipment under the teacher supervision;

In addition, for all students are prepared printed materials and laboratory instructions.

The course aims are to provide theoretical overview and the gas chromatography (GC) and related techniques applicability.

The lecture content includes theoretical and practical aspects of using GC and mass spectrometry in chemical analysis, including the retention and separation mechanisms. It also provides the possibility of applying these techniques e.g. to the sample preparation method.

Laboratory exercises allows to brings students up to dates of chromatographic techniques (GC, GC/MS), including qualitative and quantitative analysis. Running laboratory classes are dedicated to the following issues: clarifications and explanations of separation mechanisms in GC, retention setting parameters know-how, GC columns quality assurance, detection systems distinguish with the possible using for analytical purposes wide range.

Examination:

Lecture: Assessment based on exam

Lab activities: Pass unrated based on lab activities, frequency and active participation in classes.

Lecture:

The aim of this course is to acquaint students with the theoretical overview and possible to use for gas chromatography and related techniques in chemical analysis application. The lecture content includes theoretical and practical aspects of GC use.

Lecture topics:

Gas chromatography an overview.

GC apparatus part I (gases, ovens, dispensers, flow regulators, selection data station).

Optimization of GC separation process (flow, temperature gradient, qualitative and quantitative analysis - Kováts'a indexes).

GC apparatus part II (detectors).

Mass spectrometry principles.

GC/MS as a qualitative and quantitative method for compounds identifications.

MS/MS hyphenations.

Hyphenated techniques in environmental analysis.

Selective dosage (GCxGC, PTI/GC, LC-GC, GC-LC-CZE, GC-FTIR/MS, LC-MS, CZE-MS, GC-AED).

Laboratory classes:

GC / MS qualitative and quantitative analysis;

Separation process optimization (temperature and pressure gradient);

Gas chromatography evaluation of quality and applicability for retention parameters determination;

Detector’s selection (FID, ECD, DELCD, PID).

1. B. Buszewski, "Advance in separation science on the day before the 100 years anniversary of chromatography discover", SAR Pomorze, Toruń 2002.

2. B. Buszewski, "Analityka w rozwoju cywilizacji", SAR Pomorze, Toruń 2005.

3. R. L. Grob, E. F. Barry, "Modern practice of gas chromatography", John Wiley & Sons, Inc., New Jersey 2004.

4. F.W. McLafferty, F. Tureček, "Interpretation of mass spectra", University Science Books, Sausalito 1993.

5. B. Kolb, L.S. Ettre, "Static headspace-gas chromatography. Theory and Practice", Wiley-VCH, New York 1997.

6. E. de Hoffmann, J. Charette, V. Stroobant, ”Spektrometria mas”, Wydawnictwa Naukowo-Techniczne, Warszawa 1998.

7. L. Matter (Ed.), „Food and environmental analysis by capillary gas chromatography”, Hüthing GmbH, Heidelberg 1997.

8. T. Cserháti, “Chromatography of Aroma Compounds and Fragrances”, Springer, Berlin 2010.

9. Z. Witkiewicz, "Podstawy chromatografii", PWN, Warszawa 2000.

10. J. Namieśnik, Z. Jamrógiewicz, M. Pilarczyk, L. Torres, "Przygotowanie próbek środowiskowych do analizy", WNT, Warszawa 2000.

11. C. F. Poole, S. K. Poole, "Chromatography today", Elsevier, Amsterdam 1991.

Student - graduate student:

1. identifies and distinguish between issues related to the classes topic;

2. are able to find and use the required literature in English and Polish;

3. are using, applying and explaining the subject terminology related

to the study issues (not using laboratory slang) in English

and its counterparts in Polish;

4. are applying in practice the theoretical knowledge how to operate

by the apparatus and small laboratory equipment in a correct and proper way as well. Making self measurements based on the knowledge he/she gained;

5. preparing their own preparations for analysis, creating analytical procedures and standard procedures;

6. independently examines, interprets and calculates the results obtained

in the laboratory. Applying the appropriate analytical procedures. Preparing writing research reports and notes, which may be the useful for issuing the scientific publications;

7. compares, explains and describes obtained results in comparison with the available standards and literature. Predicting behavior and probable scenarios in the laboratory during sample preparation as well as the same study (e.g. principles of health and safety in the workplace).

The pass mark class is attendance at lectures and active participation, knowledge of analytical procedures is required for proper implementation

of the exercise-laboratory analysis.

Course assessment is based on the frequency and participation in the classes as well as receiving a positive evaluation at an oral, final exam.

not provided