Sample preparation in environmental analysis

The graduates of natural sciences and life sciences area

compulsory course

Number of lecture hours- 20 h

Number of laboratory classes hours- 16 h

W01: has knowledge of sample preparation in environmental analysis

W02: knows methods of sampling and sample preparation for the analysis of environmental samples

U01: skills to sampling and sample preparation using methods such as distillation, digestion, extraction (LLE, HS, P & T, TD, SPE, SPME, SFE, ASE), filtration, membrane processes

K01: set to constantly acquire new knowledge, skills and experience

- lectures as a multimedia presentations;

- one by one and on-line consultations (distance learning);

- laboratory exercises instructions prepared based on the information giving during the lectures are useful and required at the laboratory;

- individual work with the apparatus and advanced analytical equipment under the teacher supervision;

In addition, for all students are prepared printed materials and laboratory instructions.

Going to the matter of the latest developments concerning the samples preparation. The selectivity and the efficacy. Recovery and repeatability. Systems in-situ and ex situ, on-line and off-line. Hyphenated techniques, miniaturization (e.g. lab-on-chip). Obtaining the knowledge about the use of instrumental analysis techniques in environmental analysis (starting from the sampling, through the preparation method and finally to determination and validation. off-line. Techniques combined. Miniaturization, lab-on-chip. Validation.

Examination:

Lecture: Assessment based on exam

Lab activities: Pass unrated based on lab activities, frequency and active participation in classes.

Theoretical aspects of sample preparation. Sample preparation methods an overview. Trace analysis. The liquid-solid extraction process theoretical overview (SPE micro-SPE, MSPD). Mineralization (classifications, theoretical overview, methodology). Spectral methods (FES, AAS, ICP). Environmental samples analysis - new approach. Membrane techniques in trace analysis (theoretical overview classifications, characteristics, applications). Calibration. Reference materials. Sampling and sample preparation. Methods for samples isolation and enrichment. Natural products analysis. Instrumental measurement techniques (electrochemical and electromigration ones, spectroscopic, separation and others). Group analysis. Hyphenated and coupled techniques. Automatic control systems. Environmental monitoring (chemical and biological). Validation. Standards and European directives. Quality assurance.

Laboratory classes:

Going to the matter of the latest developments concerning the samples preparation. The selectivity and the efficacy. Recovery and repeatability. Systems in-situ and ex situ, on-line and off-line. Hyphenated techniques, miniaturization (e.g. lab-on-chip). Obtaining the knowledge about the use of instrumental analysis techniques in environmental analysis (starting from the sampling, through the preparation method and finally to determination and validation. This knowledge allows to work according to the good laboratory practice (GLP) rules and principles. Sample preparation techniques: gas phase analysis above the sample surface (SHS), solid-phase microextraction (SPME) - right division, partition coefficients, liquid - liquid, liquid - gas extraction systems, volatile organic compounds (VOCs) analysis, calibration methods. Mineralization as a method of sample preparation (burning, microwaves). Coulometric determination of sulfur. Heavy metals in biological samples (AAS).

1. B. Buszewski, Chromatografia i techniki pokrewne w chemii środowiska, UMK, Toruń, 1998,

2. W. Szczepaniak, Metody instrumentalne w analizie chemicznej, PWN, Warszawa, 2004;

3. J. Garaj, D. Bustin, Z. Hladky, Chemia analityczna, Alfa, SNTL Bratislava, 1989;

4. J. Namieśnik (red. nauk), Metody instrumentalne w kontroli zanieczyszczeń środowiska, WNT Warszawa, 1998;

5. Praca zbiorowa, Nowe horyzonty i wyzwania w analityce i monitoringu środowiskowy, CEEAM Gdańsk 2003

6. Praca zbiorowa, Miniaturyzacja w analityce, WPW Warszawa 2005

7. Praca zbiorowa, Nowoczesne techniki analityczne, WPW Warszawa 2006

8. J. Pawliszyn, Sampling and sample preparation, J. Wiley & Sons, New York, 2006

9. Harris, Quantitative & Qualitative Chemical Anaylysis, Freeman, New York 1999

10. C.F. Pool &S. H. Pool, Chromatogrpahy today, Elsevier, Amsterdam 1992

11. S. Mitra Sample preparation techniques in analytical chemistry, Wiley, New Jersey 2003

12. R.P. Scott, Tandem techniques, J. Wiley & Sons, New York 1998

Student - graduate student:

1. identifies and distinguish between issues related to the classes topic;

2. are able to find and use the required literature in English and Polish;

3. are using, applying and explaining the subject terminology related

to the study issues (not using laboratory slang) in English

and its counterparts in Polish;

4. are applying in practice the theoretical knowledge how to operate

by the apparatus and small laboratory equipment in a correct and proper way as well. Making self measurements based on the knowledge he/she gained;

5. preparing their own preparations for analysis, creating analytical procedures and standard procedures;

6. independently examines, interprets and calculates the results obtained

in the laboratory. Applying the appropriate analytical procedures. Preparing writing research reports and notes, which may be the useful for issuing the scientific publications;

7. compares, explains and describes obtained results in comparison with the available standards and literature. Predicting behavior and probable scenarios in the laboratory during sample preparation as well as the same study (e.g. principles of health and safety in the workplace).

The pass mark class is attendance at lectures and active participation, knowledge of analytical procedures is required for proper implementation

of the exercise-laboratory analysis.

Course assessment is based on the frequency and participation in the classes as well as receiving a positive evaluation at an oral, final exam.

not provided