Fundamentals of bioanalytics

Elementary requirements - basics of chemistry and biology at the high school level.

Prerequisites - the ability to independently search and use available sources of information and use scientific literature.

elective course

Hours implemented with teachers ( hrs):

- participation in lectures (30 h)

Time devoted to individual student work (hrs):

- preparation for lecture (5 h)

- reading the literature (10 h)

- preparation for credit (15 h)

W1: Has a basic knowledge of chemistry and biology, its development and importance for the progress of science and life sciences and the knowledge of the world and the development of mankind.

W2: Describes the basic processes of biochemistry, metabolism in the human body.

W3: Characterizes the methods of determination of biologically active compounds.

U1: The student uses the basic knowledge of bioanalytics, knows the methods of sample preparation and determination techniques, is able to describe the phenomena occurring in the human body,

U2: Analyzes the acquired knowledge of bioanalytics from various sources and is able to argue and defend his/her position.

U3: Acquires proficiency in performing calculations on the basis of data obtained from measurements by instrumental methods.

K1: Is aware of the threat posed by xenobiotics to all living organisms, makes independent and sometimes difficult decisions regarding the acquired knowledge; is able to independently search for information in popular scientific literature related to environmental chemistry.

K2: He has the ability to work independently and effectively with a large amount of information regarding the dangers of toxic substances in the environment, perceives the relationships between phenomena and correctly draws conclusions using the principles of logic.

K3: The student is oriented to continuous acquisition of new knowledge, skills and experience; sees the need for continuous improvement and enhancement of professional competence; knows the limitations of his own knowledge and understands the need for further education.

Conventional lecture (multimedia presentations) combined with discussion

- display

- informative (conventional) lecture
- problem-based lecture

- classic problem-solving

To learn the theoretical basis with a focus on the practical use of analytics in chemistry, biology, biochemistry, medicine and toxicology. To acquire basic knowledge of a modern analytical laboratory. Acquisition of the ability to choose the correct method of collection and preparation of biological, medical and pharmaceutical samples. Correct performance of measurements and analysis, reports, good laboratory practice. Quality assurance, validation.

Theoretical foundations in medicinal chemistry, drug chemistry, food chemistry. Historical outline. Terminology. Structure and properties of the cell. Characteristics of lipids, carbohydrates, proteins, enzymes, receptors, nucleic acids. Basics of proteomics, genomics and metobolomics. Structure of DNA and RNA. Antibodies. Chirality. Theoretical basis of selected chromatographic, electromigration, spectroscopic, radiometric, immunological techniques, mass analyzers, ion detectors. Modern methods of analysis of bio-molecules. Specific methods of sample preparation. Criteria of operation of research and clinical laboratories. Quality assurance systems. Accreditation and certification of laboratories. Analytical procedures and their validation. Statistical evaluation of results.

P. Kafarski, P. Wieczorek, Ćwiczenia Laboratoryjne z Chemii Bioorganicznej, Wydawnictwo Uniwersytetu Opolskiego, Opole 1997

Cygański, Metody spektroskopowe w chemii analitycznej, WNT, Warszawa 1997

Z. Witkiewicz Podstawy Chromatografii, WNT, Warszawa 2000

W. Szczepaniak, Metody instrumentalne w analizie chemicznej, PWN Warszawa 2005

Nowoczesne techniki analityczne, praca zb. pod red. M. Jarosza, Oficyna Wyd. PW, Warszawa 2006

H. Waldmann, P. Janning, Chemical Biology. A practical course, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim 2004

Proteomika, praca zb. pod red. A. Kraj, J. Silberring, Wydzial Chemii UJ, Kraków 2004

Spektrometria mas, praca zb. pod red. P. Sudera, J. Silberringa, Wyd. UJ, Kraków 2006

Mikrobioanalityka, praca zb. pod red. Z. Brzózki, Oficyna Wydawnicza PW, Warszawa 2009

S. R. Mikkelsen, E. Cortón, Bioanalytical Chemistry, Wiley-Interscience, Hoboken, New Jersey 2004

L. Huber, Dobra praktyka laboratoryjna, PIOŚ, Warszawa

Assessment methods:

- Activity, written work or presentation, other form determined at the first lecture

Grading criteria:

Lecture: credit on the basis of activity, written work or presentation

ndst- less than 8 points (less than 40%)

dst- 8-11 pts (40-55%)

dst plus- 11.2-13 pts (56-65%)

db- 13.2-14.8 pts (66-74%)

db plus-15-16 pts (75-80%)

bdb- 16.2-20 pts (81-100%)

not applicable