Hyphenated Separation Techniques

General issues of advanced chromatographic and electromigration techniques

Lecture:

Total student workload Contact hours with teacher:

np.

- participation in lectures - 30 hrs

- consultations - 15 hrs

Self-study hours:

np.

- preparation for lectures - 15 hrs

- writing essays/ papers/ projects - 5 hrs

- reading literature - 5 hrs

- preparation for test/ examination - 30 hrs

Altogether: 100 hrs (7 ECTS)

Laboratory:

Contact hours with teacher:

np.

- participation in lectures - 30 hrs

- consultations - 15 hrs

Self-study hours:

np.

- preparation for lectures - 15 hrs

- writing essays/ papers/ projects - 5 hrs

- reading literature - 5 hrs

- preparation for test/ examination - 30 hrs

Altogether: 100 hrs (7 ECTS)

Lecture:

W01: uses and explains terminology specific to the subject in English and its equivalent in Polish - K_W01

W02: recognizes and distinguishes between issues related to the subject of classes - K_W13, K_W14

Laboratory:

W01: uses and explains terminology specific to the subject in English and its equivalent in Polish - K_W01

W02: recognizes and distinguishes between issues related to the subject of classes - K_W13, K_W14

Lecture:

U01: apply theoretical knowledge in practice, including the use of facilities and other small laboratory equipment in a correct and proper way - K_U11.

U02: make measurements - K_U11.

U03: prepare on their own preparations for the tests, analytical procedures - K_U14.

U04: can analyzes, interprets and calculates the results obtained in the laboratory, in the course of his work - K_U11.

U05: can develop analytical methodology - K_U14

Laboratory:

U01: apply theoretical knowledge in practice, including the use of facilities and other small laboratory equipment in a correct and proper way - K_U11.

U02: make measurements - K_U11.

U03: prepare on their own preparations for the tests, analytical procedures - K_U14.

U04: can analyzes, interprets and calculates the results obtained in the laboratory, in the course of his work - K_U11.

U05: can develop analytical methodology - K_U14

Lecture:

K01: apply appropriate analytical procedures - K_K02.

K02: draw up test reports and notes, which can be used as a basis for the preparation of scientific publications - K_K05.

Laboratory:

Learning outcomes - social competencies K01: apply appropriate analytical procedures - K_K02.

K02: draw up test reports and notes, which can be used as a basis for the preparation of scientific publications - K_K05.

Expository teaching methods:

- informative lecture

- lectures with discussion

Laboratory experiments

The aim of course is to familiarize students with the theoretical basics and possibilities of the application of modern methods, especially chromatographic techniques in analytics.

Sample preparation using solid phase extraction (SPE) and quantitative analysis by high performance liquid chromatography (HPLC).

Application of GC/MS coupled technique.

Separation of nanoparticles by field-flow fractionation - FFF.

Determination of inorganic ions by ion chromatography.

The determination of volatile organic compounds (VOCs) by use of SPME-GC, P&T-GC and LLE-GC methods.

The comparison of HPLC and UHPLC techniques.

Miniaturized separation techniques: capillary liquid chromatography (nanoLC) and capillary electrophoresis.

1. B. Buszewski, E. Dziubakiewicz, M. Szumski [red.], Electromigration techniques, Springer-Verlag, Berlin Heidelberg 2013.

2. J.R. Dean, Methods for environmental trace analysis, John Wiley & Sosns Inc., Chichester 2003.

3. B. Ardrey, Liquid chromatography-mass spectrometry: an introduction, John Wiley & Sosns Inc., Chichester 2003.

4. I. Baranowska [Ed.], Handbook of Trace Analysis Fundamentals and Applications, Springer International Publishing 2015.

5. P. Bocek, Analytical capillary isotachophoresis, Verlag Chemie, Weinheim 1987.

6. S. Mitra, Sample preparation techniques in analytical chemistry, Wiley, New Jersey 2003.

7. R.P. W. Scott , Tandem techniques, J. Wiley & Sons, New York 1998.

8. C.F. Poole, The Essence of Chromatography, Elsevier, Amsterdam 2003.

9. J. Pawliszyn [Ed.], Sampling and sample preparation for field and laboratory Elsevier Science B.V., Amsterdam 2002

10. C. F. Poole, S. K. Poole, Chromatography Today, Elsevier, Amsterdam 1991.

11. J. Pawliszyn, Solid Phase Microextraction, Wiley & Sons, New York 1997.

12. D. C. Harris, Quantitative & Qualitative Chemical Analysis, Freeman, New York 1999.

To pass of laboratory experiments is the knowledge of analytical procedures required for the correct execution of exercises-laboratory analysis, a positive evaluation of the colloquium preceding the exercise include the written report for each experiment – W01, W02, K02

fail - 2 pts (≥50%)

satisfactory - 3 pts (50-60%)

satisfactory plus - 3,5 pts (61-65%)

good - 4 pts (66-75%)

good plus - 4,5 pts (76-80%)

very good - 5 pts (≤81%)

Work placement not applicable